邢唷> FHE欹W 餜V bjbjD蒁5&&Ｃe&Ｃe*i!N N www8$+b'(((+++++++$.06*+Ew(("("((*+'uo+***(8'8+*(+**_*@〈?U)**+0+*1*@1*1w*0((*(((((*+*+*(((+((((1(((((((((N O : D桍N2 慹倐鵞~喢O'`z伬o }蕬>e纎 }剉MQ玼歔MO蔛導c:g6RR 闂 刟,b hg=N讒a hgvQl_a 臑鷁NSa hT裇梘a _lN5峚, * a淨N钀WSwmnND崘n_裇)R(u蛻筽瀃寶 WSwm4lxvz@b -N齎4l褃f[xvzb ^轢 510300 -N齎 b Nwmwm m'Yf[ 4lNu}Tb/gf[b Nwm 201306 Xd亯:N哊穬鍂肙'`z伬o }蕬>e纎 }(GnRH)導c:g6R 錘縊(WA~瞼-N鬴}Y擽(u ,g瀃寶)R(uMQ玼膥S筫誰xvz哊GnRH(W慹倐鵞~唘S鈃裇瞼擭*N鰁gII髞VIg -N(W^y蟸鹼邁蔛uS鈃-N剉MQ玼歔MO孴R^ 錘蔛(W潃饊z伿SuS鈃-NGnRH酧鱏愶嶘vsQ剉導c郪 }c-Jun蔛uS臑藛}v烻剉h埦從墜_0觺済>f:y慹倐鵞~哵y蟸鹼邁蔛uS鈃-NX[(WGnRH-I{|f:y儚:_剉MQ玼3'` HT皊貧h埦4ls^0歔蠎PCR觺済>f:yl廢_郪P[c-Jun(WIIguS鈃蔛潃饊z亜vh坴^wQ gyr歔MQ玼歔MO GnRH-I鵞uS臑藛}v烻剉導c\O(u颯齹/f惽徝O蹚c-Jun剉h埦徫N 導cuS臑藛}v烻剉飝/}0鍕xvz:N栶廏nRH剉R蘬導c:gt 駇eQ哊銐慹倐鵞~唘S鈃裇瞼導c:g6R`Y歔哊鶺@x0 sQ.曂GnRH^y蟸鹼邁uS鈃慹倐鵞~; MQ玼膥SuS臑藛}v烻qPCR The identi鹀ation and distribution of gonadotropin releasing hormone in the central nervous system and ovary of each stages in Penaeus monodon HAN Ping, YANG Lishi, YANG Qibin, HUANG Jianhua ZHOU Falin JIANG Shigui (South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300) Abstract: Gonadotropin-releasing hormone (GnRH) plays important roles in reproduction. In the present study, we demonstrated the existence of GnRH-like peptides in the central nervous system (CNS) and ovary of each stage in Penaeus monodon using immunocytochemistry and quantitative real-time PCR of c-jun and vitellogenin expression quantity. The immunoreactivity (ir) of GnRH was detected in the of medium-sized neurons located in deutocerebrum, both medium-sized and large-sized neurons in thoracic ganglia and abdomin  >DHNVX`bjltv~ & ( * , 钷夷婪畽坵gwg坵g[繫jh$UmHnHuh'CJOJQJaJh'CJOJQJaJmHsH!h'CJOJQJaJmHo(sH!h'CJH*OJQJaJmHsHh'OJQJh'OJQJmHo(sHh'H*OJQJh'OJQJo(h'h'5丆J QJ\乤J o(h'OJPJQJ^Jh'CJ OJPJQJ^JaJ !h'CJ OJPJQJ^JaJ o(  @D( * J L *rt $:`:gd' $d橚a$gd' $:`:a$gd'$a$gd'gd', 2 L X   ( d p     R X h r t x  H L T \ v z *蹯莒造造造造造造造造造造造造造造造匀越唇穿詾挒妴妴h'h'CJaJh'56丆J \乤J h'5丆J \乤J h'5丱JQJ\h'OJQJ\h'OJQJ\乷(h'5丱JQJ\乷(h'OJQJh'OJQJfHq h'OJQJo(h'5丵J\乷(2q4t "&(,.248:<@Bvxz瘐铛瘐殁橄壤祭祭祭籍ぜ搥搊_h'B* CJOJQJo(ph3!h'5丅*CJOJQJ\乸hf$h'5丅*CJOJQJ\乷(ph3f!h'5丅*CJOJQJ\乸h3fh64OJQJ^Jjh$UmHnHuh64jh64U h'hy{Q$h$h'CJ OJPJQJ^JaJ h'6乗 h'\U h'6h' h'5乗al ganglion. In addition, ir-GnRH was observed in thsion in stage II, the highest expression of vitellogenin in stage IV. This 鹡ding provides supporting data that ir-GnRH-like peptide may exist in Penaeus monodon, as well as may has a direct and indirect role in promoting ovarian maturation in the early stage. Key words: GnRH, CNS, ovarian maturation, Penaeus monodon, immunocytochemistry, qPCR      ,gu:N 2018t^-N齎4lf[Of[/gt^O簨噀Xd亯!jg 1.蟢莧簨噀Xd亯R:N-N0駛噀$N钀R N譙瘇駛噀 ;` N厤菑A4NugY N梍厤菑,g!jg鵞擽剉W[pe 0 2.鲖 cgq,g!jg剉u樄忀0W[SO0W[鱏蔛祂=勽曒宼e╜剉Xd亯@BxzRF   P R T V G$gd'PR 24DF    @ B L N P T V 襻襻卫伟吾襻襻Β巭巭巔 h'hy{Qh64CJOJQJ^JaJh'CJOJPJQJ^JaJ'jh'CJOJPJQJU^JaJh64h'B*CJphh'0JB* OJQJo(ph3h640JB* OJQJph3$jh640JB* OJQJUph3h'B* CJOJQJo(ph3h'B* CJOJQJph3309182P皞. 捌A!"#悹$悹%癝班 惄w666666666vvvvvvvvv6666>6>66666666666666666666666666666666666666666666666>6hH66666.66666666666666666666666666666666666666666666666666666666666p62&6FVfv2(&6FVfv&6FVfv&6FVfv&6FVfv&6FVfv&6FVfv8XV~ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@6666 OJPJQJ_HmH nHsH tHZ`Z 奪ck噀 $1$a$0CJKHOJPJQJ^J_HaJmH nHsH tH$A $ 貫祂=刉[SOBiB 0nf恏U`!> 奪0厤 >*B*CJOJQJ^JaJph616 奪pstyle27 style448/A8 奪pstyle6115丅*\乸hF/QF 奪pstyle6215丅*OJQJ\乛Jo(ph: @b: '0u9r G$a$CJaJ:/q: '0u W[&{CJOJPJQJ^JaJPK!檗[Content_Types].xml瑧薔0E鱄鼉-J湶@%閭菐洽|廊$韶钵UL襎B l,3鳛;鉹得槣B+$G]ミ7O侪V墎4憄o j!?涝鉛4(垂爯逧"皯煜恪庪r櫈`蕼sn彻脄丹_C n执镅yh"cAl>wc:裁 6l烡亷龍@"&63yy@哑t!豀鏖jpVТ,ye[ry3鹲:FXI 杩!!塏峪u;u =筫Aｈv荈:轗藳1鞭L;+ 藩踡徣/4媙b窼謠讃諂^7菹Ky鍠恢d钞额崞濢楶s妛冠約hJ J;躔姵'筰囼N $F势墮鴦垹)l駤畉2岍 w梦_ [}K<潊{l<嬺4幠r监g沭!tズ|塑+醢 m邌6橧!Q] 〨s殔刏賲皑[X预鸈X,+発r`p}L#櫑$諎飒d^d7莹谻,*`欓轰簈yruI! 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